Progressive neurodegeneration within interconnected brain regions is a common hallmark of several CNS diseases, such as Alzheimer’s disease, Parkinson’s disease, and amyotrophic lateral sclerosis (ALS). To be able to mimic the spreading of neuropathologies from brain region to brain region poses a particular challenge in CNS disease modelling. Additionally, the absence of high-capacity platforms, reflecting the complexity of progressive neuropathologies and that are suitable for intervention approaches, represent the current bottleneck in disease research and drug discovery campaigns.
To model the spreading of neuropathologies, we have developed a novel microfluidics platform which we combine with our previously established optical electrophysiology technology to create unique high throughput in vitro assays that reflect the hallmarks of the neurodegenerative disease cascade.
In this presentation, we will discuss the design concept and development of this new microfluidic assay platform including a technical validation of the platform. Furthermore, we will show how the platform can be used to model key aspects of Alzheimer´s disease associated neuropathology in primary neuronal cell cultures. We present future outlooks to apply our microfluidic assay platform for in vitro disease modelling for neuromuscular disorders, such ALS and muscular dystrophies, as well as for chronic pain research.
Tuesday March 19 @ 4 pm CET / 11 am EDT / 8 am PDT