Innovative in vitro assays with enhanced translational value
Our discovery platform enables the development of assays for high capacity testing of changes in excitability and synaptic communication in a wide range of in vitro models. This can be used not only for screening large number of compounds, but also to efficiently identify optimal assay parameters and thereby maximize translatability.
Our primary cell assays fill the gap between less complex model systems, such as high throughput screening in over-expressing cell lines, and in vivo animal models, and are enabling our Clients to prioritize moving the most effective molecules into the clinical phase.
All aspects of the company’s collective experience, from engineering and product development to drug discovery, are integrated when devising and creating new and innovative assays for our Clients.
Accelerated decision-making through customized services
Stimuli and read-outs
In vitro models for central and peripheral pain
Our models for peripheral pain identify compound effects on nociceptor excitability and morphology in neuronal cultures. They are based on in vitro sensitization of sensory neuronal cultures from either rodent dorsal root ganglia (DRG) neurons, trigeminal ganglia neurons, or human iPSC-derived peripheral neurons. The models are responsive to many pain-relevant mechanisms, as assessed by the pharmacological action of clinically relevant compounds (e.g. raloxifene, duloxetine, sertraline and gabapentin), and enable identification of novel targets and compounds using both target-based and phenotypic approaches.
For central pain, our models are based on neuronal cultures from either brain regions or spinal cord with integrated neuronal circuits for functional and morphological screening. Engaging central and peripheral models in parallel may provide insight whether the compounds have a peripheral or central site of action.
The Cellectricon team is experienced in devising and creating new models, as well as adjusting established models to encompass new aspects. Since increasing amounts of literature support the understanding that inflammatory mediators play a part not only in inflammatory pain, but also in neuropathic pain, we are increasing our efforts in neuroinflammation research. We are, for example, exploring the role of anti- and pro-inflammatory cytokines and their role in chronification of pain.